Author(s):
1. Irena Kuzmanović, Radman, Republic of Srpska, Bosnia and Herzegovina
2. Aleksandra Đeri, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina
3. Adriana Arbutina, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina
4. Ognjenka Janković, Republic of Srpska, Bosnia and Herzegovina
5. Nataša Trtić, Republic of Srpska, Bosnia and Herzegovina
6. Vladan Mirjanić, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina

Abstract:
Introduction: Hard dental tissues represent ideal tissues for assessing the long-term effects of exposure to toxic metals. The aim of this paper was to determine the abundance and the relation between mass portions of the following elements: magnesium, carbon, oxygen, sodium, aluminium, phosphorus, chlorine, potassium, iodine and lead in certain parts of the teeth(enamel, enamel-dentine line, dentine, pulp) with rats with experimentally induced diabetes mellitus (DM) by using SEM/EDS analysis, after 14 and 30 days of exposing animals to lead. Material and methods of work: The study was conducted in rats of Wistar strains divided into three groups. The first group consisted of 8 rats with experimentally induced DM, taking lead in the course of 14 days at a concentration of 1500 ppm; the second group consisted of 8 rats taking lead in the course of 30 days at a concentration of 1500 ppm, while the third control group consisted of 5 healthy rats. The teeth samples were analysed by scanning electron microscopy (SEM). Results: No lead was detected in the teeth of rats that received lead in drinking water in the course of 14 days, while the average values of mass portions of other examined elements amounted to: carbon -24,25 %, oxygen – 38,17%, sodium -0,9%, magnesium -0,11%, aluminium – 0,07%, chlorine – 0,21% and iodine – 0,32%. The average values of the mass portions of examined elements in the enamel of teeth of rats receiving lead in the course of 30 days amounted to: lead -0,36%, carbon-31,09%, oxygen – 41,13%, sodium – 0,91%, magnesium – 0,21%, chlorine – 0,22%, potassium – 0,03% and iodine – 0,17%. Conclusion: Mass portions of elements found in the teeth enamel of rats receiving lead in the course of 30 days through drinking water were slightly higher but without statistically significant difference in relation to the mass portions of elements in the group of teeth of rats taking lead through drinking water in the course of 14 days. The lead was detected in the teeth of rats that received lead for 30 days, and only in theenamel.

Key words:
mass portions of elements in the enamel, dentinogenesis, SEM/EDS analysis

Thematic field:
SYMPOSIUM B - Biomaterials and nanomedicine

Date of abstract submission:
27.06.2018.

Conference:
Contemporary Materials 2018 - Savremeni Materijali

Abstract